|Contributions||Brunel University. Department of Applied Biology.|
|The Physical Object|
|Number of Pages||216|
Electron microscopy was performed with the CM12 transmission electron microscope (Philips Electronic Instruments, Inc., Mahwah, N.J.) at 80 kV. Phage DNA isolation and restriction analysis. The Lactobacillus phages were purified from 1 liter of mitomycin-induced lysate by a procedure described by Maniatis et al. Cited by: The presence of a plaque means that: i) the tested sample contains phages; ii) the phage is virulent against the tested strain; iii) the strain is sensitive to the phage. Each phage particle that gives rise to a plaque is called a plaque-forming unit (PFU). One plaque corresponds to a single phage particle and phages can easily be by: 5. mission electron microscope (TEM) is mainly now used by microbiologists for the study of the intracellular or cell wall struc-ture , or for a quick examination of the bacterial cell with negative staining. The scanning electron microscope (SEM) is used for the study of the cell surface and its pro-perties [1, 5, 6, 19]or in the greater field of. Post isolation, the φ phage would only form plaques providing the agarose concentration was % or less (Figure 1). Based on these findings it would seem prudent to consider using % agarose in screening studies for hosts for new phages. In marked contrast the coli-phage T4 produced plaques at all agarose concentrations tested.
Negatively stained preparations of macromolecular assemblies composed of lecithin, cholesterol and saponin have been examined by electron microscopy. Studies were made of the effects of different negative stains, pH and fixatives on the self‐assembly process that gives rise to helical and related structures, on the characteristic appearance. Diagnosis of Platelet Disorders by Electron Microscopy. Hilary Christensen. Division of Haematology/Oncology, Program in Cell Biology, The Hospital for Sick Children, Toronto, Ontario, Canada. Book Editor(s): John W. Stirling. Centre for Ultrastructural Pathology, IMVS – SA Pathology, Adelaide, Australia. It was not until the electron microscope became available that insights into relationships among platelet structure, function, and pathology began to evolve (8, 9). Even then, the problems of fixing platelets to preserve their fine structure and relate physical changes following activation to their role in hemostatic physiology took. Comprehensive platelet aggregation studies are a standard and accepted part of any coagulation work-up following initial screening tests, PT/INR, aPTT and von Willebrand factor testing. Platelet function testing is an important part of the evaluation of common and rare bleeding disorders [1, 2, 3, 8].
In book: Lactic Acid Bacteria, pp Phage morphology remained undetermined until electron microscopy became available during the s. Nevertheless, phages attacking Lactococcus lactis. In book: Lactic Acid Bacteria: Microbial and Functional Aspects (pp) has been demonstrated by immunogold labeling and electron microscopy. Expression of ltp in prophage- and plasmid. Electron microscopy. A ml sample of phage lysate (titer of at least 10 9 PFU/ml) was centrifuged at 23, × g for 1 h at 4°C. The supernatant was removed, leaving approximately μl in the tube. The phage pellet was washed twice with ml of ammonium acetate ( M, pH ). Research on lactic acid bacteria (LAB) has advanced significantly over the past number of decades and these developments have been driven by the parallel advances in technologies such as genomics, bioinformatics, protein expression systems and structural biology, combined with the ever increasing commercial relevance of this group of microorganisms.